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The main environmental impact is due to the cultivation phase while the transformation has limited consequences.
The study, supported by the European Union (project OPTIMA - Optimization of Perennial Grasses for Biomass Production), was developed and applied to the cultivation phase of several perennial crops, in marginal soils of the Mediterranean region, using environmental impact assessment (EIA) protocols.
It consists of three distinct phases: (i) metabolic engineering to create the evolutionary pressure on the applied strain followed by (ii) a cultivation phase with growth as straightforward screening indicator for the evolutionary event, and (iii) comparative whole genome sequencing (WGS), to identify mutations in the evolved strains, which are eventually re-engineered for verification.
In the coconut cultivation phase, chemical processes for the production of fertilizers, the utilization of these substances, physical infrastructure for coconut cultivation, and emissions from fertilizers and irrigation are included in the system boundary.
Table 1 Yield coefficients during the fed-batch process of the yeast cultivations in triplicate Cultivation phase YX/G (gbiomass/gglucose) Batch phase 0.31 ± 0.01 Controlled feeding phase 0.49 ± 0.01 Entire cultivation 0.46 ± 0.02 The values in the table are mean value ± standard deviation The starting time of the controlled feeding phase is shown in Fig. 3d.
Intercropping maize in tobacco and sugarcane enhanced utilization of land space and physical resources during the late growing period of tobacco and early cultivation phase of sugarcane, adding a season of maize production.
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Taking advantage of next-generation sequencing combined with cluster, correlation and differential expression analyses, we could identify 89 different miRNAs, which were differentially expressed in the different cell lines and cultivation phases.
In some cases the flasks were inoculated in a time-displaced way to collect samples of all cultivation phases.
The OSP supplementation considerably responded to different cultivation phases.
Changes in hyphal growth and morphology were analyzed at distinct cultivation phases using automated image analysis.
For the other cultivation phases, the C-balances did not close to 1.0.
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