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One of the major factors affecting large-scale algae technology application is poor wintering cultivation performance.
However, as the agitation speed was increased from 300 to 400 rpm, the cultivation performance of P. acidilactici was declined.
For facultative microorganisms such as P. acidilactici, facultative condition was shown to improve the cultivation performance of P. acidilactici compared to anaerobic condition (Fig. 3).
In order to achieve high algal cultivation performance, the optimal structural parameters of the bioreactor were determined using computational fluid dynamics (CFD) simulation.
The cultivation performance of P. acidilactici in the constant fed-batch fermentation coupled with extractive fermentation using the condition of dispersed anion-exchange resin were conducted at feeding rate of 0.015 L/h.
Further improvement in the cultivation performance of P. acidilactici in fed-bath fermentation with in situ addition of anion-exchange resin significantly helped to enhance the growth of P. acidilactici by reducing the inhibitory effect of lactic acid and thus increasing probiotic production.
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On the basis of the macroscopic cultivation performances, the analyses of intracellular metabolite profile and the mycelium morphology, as well as the further verification of sodium gluconate fermentation, 300 g/L glucose was selected as the optimal initial concentration of seed culture, which was more suitable for the sodium gluconate fermentation.
This work describes the characterisation and culture performance of a novel, orbitally shaken, single-use photobioreactor (SUPBr) system for microalgae cultivation.
Table 1 summarises the data for the cellular performance indicators from all the cultivations performed.
Analysis of ethanol, glycerol and acetate from the cultivation was performed by high performance liquid chromatography (HPLC) using a Dionex Ultimate 3000 HPLC unit (Thermo scientific, Dionex Corporation, Sunnyvale, USA) equipped with an Aminex HPX-87H (Biorad, USA) column of length 300 mm and diameter 7.8 mm, which was packed with 9 μm particles.
Such alleles are good candidates for being functionally important to domestication, cultivation or agronomic performance, or in linkage disequilibrium with such genes.
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