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It was found that biomass formation and PHB accumulation were a function of various cultivation parameters.
Multivariate experimental design and quantitative bioprocess development methodologies were used to identify the key cultivation parameters for PHB accumulation.
To this end, different cultivation parameters including substrate concentration and operation modes (batch and fed-batch) were evaluated.
The optimal cultivation parameters for A. niger were determined using full factorial and steepest ascent experimental designs.
Optimum cultivation parameters were (g/L): 12.7 of lactose, 16.7 of tapioca, 1.8 of ammonium sulfate and 8.0 of peptone.
The parameters of the kinetic model represent the physiological effects that the cultivation parameters impose on the organism.
In the next step, cultivation parameters such as pH, temperature and moisture content were optimized using Box-Behnken design.
Bacillus methanolicus MGA3 cultivation parameters.
Five of these are combinatorial cultivation parameters.
Here, the cultivation parameters form the predictors.
Optimization of various cultivation parameters plays an important role.
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