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The most appropriate cultivation methodologies (i.e. floating long-lines vs. bottom cultivation) for Gracilaria production were also evaluated.
For these reasons, we here raise concerns for the conservation of both species of Cynomorium and strongly voice the need to develop cultivation methodologies.
However, current microalgal species and cultivation methodologies are thought to hamper production of algae-based energy in an economically viable manner [ 1, 4].
Although this explanation seems coherent, the literature is somewhat ambiguous, as both increases and decreases in glycerol yield have been observed following addition of furfural and/or HMF to batch and chemostat cultivations, a fact that is likely the result of different cultivation methodologies and strain-specific differences [ 15, 38- 40].
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Concerning the protection of non-target organisms that might be harmed due to GMP cultivation, a methodology was developed to classify the susceptibility/sensitivity of nature reserves [NSG] in Germany as being part of the receiving environment that might be affected due to GMP cropping in their vicinity.
One way to overcome this limitation is by improving cultivation-based methodologies [ 4, 5].
In order to bypass the limitation of cultivation-based methodologies, metagenomic approaches became a powerful tool to directly study the diversity of genes within microbial communities, analyze their biochemical activities, and prospect novel biocatalysts from environmental samples [ 19- 22].
By using this medium, an experimental maximum protease activity of 280.72 U/ml in 7 days cultivation verified the applied methodology.
Thus, the analysis of the GENOSOJA database would be best complemented by next-generation sequencing experiments to replace the SSH methodology and cultivation in pot systems, instead of under hydroponic conditions.
The study of pig macrophage biology has recently been expedited by the completion of a draft genome sequence [ 14], comprehensive annotation of the pig immunome [ 9], the development of a comprehensive expression array platform [ 15], methodology for cultivation of macrophages [ 16] and identification and characterisation of subsets of monocytes [ 17].
The lipid production was studied according to the experimental design methodology, for different cultivation conditions for agitation and aeration.
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