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Contaminants may not directly affect the production of algae but they may affect the pH, which may lead to cultivation limitations.
Although today's studies of microbial diversity are no longer prone to cultivation limitations of 1985, they are still predominantly framed around operational, technique-based definitions rather than a deep understanding of the underlying biology.
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The workability soil quality SQ7 includes physical hindrance to cultivation, and limitations to cultivation imposed by texture/clay mineralogy.
Historically, one of the main challenges in algae cultivation is CO2 limitation.
In this work, carbohydrate-enriched biomass from cyanobacteria was obtained by photoautotrophic cultivation under nitrate limitation.
This suggests an improvement in pentose uptake rate through evolution and confirms that continuous cultivation under substrate limitation tends to generate an evolved population with improvement at the level of nutrient transport [ 12, 13].
Synechocystis sp. PCC 6714 was studied under various cultivation conditions and nutritional limitations.
However, heterotrophic cultivation also has some limitations.
Recently, a medium optimization study showed phosphate limitation in continuous fermentation increased fatty acid yield and biomass-specific productivity compared to carbon-limited cultivation (Youngquist et al., 2013).
Although nitrate exhaustion over cultivation resulted in nitrate limitation, growth of the microalgae was not reduced.
Based on the demand not to fall short of 80% cell viability for the production, EPO could be produced for 2 days (30%) longer compared to the control due to a more economic exploitation of glucose, and the prolonged viability period of the cells using a batch cultivation driven by glutamine limitation.
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