Sentence examples for cultivation integrated from inspiring English sources

So far no cultivation integrated foam fractionation process for rhamnolipid production has been reported.

This led to an unforeseeable effective method to produce and in situ concentrate rhamnolipids via simple cultivation integrated foam fractionation using the heterologous production strain P. putida KT2440 containing a plasmid for mono-rhamnolipid production in a bioreactor.

It was previously shown that simple cultivation integrated foam fractionation is an effective tool for biosurfactant production e.g. of surfactin using Bacillus subtilis (Chen et al. 2006; Willenbacher et al. 2014, 2015) or HFBII using Saccharomyces cerevisiae (Winterburn et al. 2011).

In this article a cultivation integrated foam fractionation process for rhamnolipids in a bioreactor using P. putida KT2440 as a heterologous production strain is described with low biomass enrichment in the foamate giving the opportunity to remove highly concentrated rhamnolipids from the cultivation broth in situ.

However, cultivation integrated foam fractionation in rhamnolipid production was shown to be not feasible due to highly concentrated biomass in the foam leading to the necessity to develop solutions regarding cell retention e.g. using magnetic separation or cell recycling (Gruber 1991; Heyd et al. 2011; Küpper et al. 2013).

However, taking advantage of the non adhesiveness of the cells this article presents an effective simple cultivation integrated foam fractionation method to produce and in situ concentrate rhamnolipids using a heterologous production strain independent of pH, temperature and defined medium composition with little cell accumulation in the foam.

Isolated monoclonal strains were grown competitively in either T25 cell culture flasks in an Infors Minitron Incubator (27 strains) or in a 96-Deepwell plate (81 strains) in a custom build automated cultivation chamber integrated into a liquid handling robot.

We used a custom screening platform, based on 96-deepwell microplate culturing in an in house designed cultivation chamber integrated in a liquid handling robot for screening and selection; in addition we also used a more conventional system.

In this study, fungal and engineered bacterial cultivations were integrated with a modified anaerobic digestion to accumulate fatty acids on sewage sludge.

However, a nationwide information system on spatial and temporal cultivation patterns integrating agricultural data of all federal states has not been established, yet.

This precise simulation of growth was further confirmed with dFBA, which was used to describe the dynamics of growth in batch cultivation by integrating standard steady state FBA calculations into a time dependent function of biomass accumulation and carbon source depletion.