The extraction of a threefold volume of lava granules in comparison to the volume used for preparation of Fig. 2 as well as the additional cultivation in batch-cultures increased the total protein yield and enabled us to detect the proteins in the resulting gels with colloidal CBB stain (Fig. 5).
The validamine addition at the beginning of cultivation in fed-batch fermentation by A. utahensis ZJB-08196 accelerated the accumulation of acarbose greatly.
In this setup, cultivation was conducted in batch mode, then a portion of the culture liquid was withdrawn, and the same volume of the production medium was added.
The microalga Scenedesmus obliquus was cultured in two cultivation stages: (1) in batch with real wastewater; (2) maintaining the stationary phase with different conditions of CO2, light and salinity according to a factorial design in order to improve the lipid content.
Either the inhibitors have been included in the cultivation media initially in batch cultures or they have been pulsed during the exponential or stationary growth phases [ 8, 26- 28].
To improve the microalgae production in batch cultivation, a cultivation mode that continuously pre-harvesting Spirulina platensis from photobioreactor (PBR) with culture medium recycling was proposed.
This occurred 21-22 hours after start of the aerobic batch cultivation in the various cultivation batches.
The pronounced differences in specific productivity were observable during continuous T25 and spinner flask cultivation as well as in batch culture experiments.
This coincided with an up regulation of the putative gene encoding for alcohol dehydrogenase (Pc21g22820) during chemostat cultivation, preceding the regrowth in batch culture.
Cultivations were conducted in batch or pulse-fed batch mode (as indicated in Figures and Tables), the dissolved O2 concentration was >30%% of air saturation and mechanical stirring was performed with two Rushton-type impellers, in the range of 200 400 rpm.
Main cultivations were run in batch or fed-batch mode in benchtop bioreactors (Labfors III, Infors AG, Bottmingen, Switzerland) with 2 L as working volume.
