After 4 h of co-cultivation, gaps formed underneath >99% of the MCF-7 tumour spheroids (gap area was on average ∼1.15 × 10 μm) whereas no or only small gaps were formed underneath normal lung fibroblasts.
During the continued-cultivation, the newly generated nanocellulose will continue to grow into the cultivation vessel through the gap between the two half-pipes and finally fills the whole space of the vessel.
Indeed, 3D cell culture is one favorable approach for reducing the gap between artificial cultivation in vitro and the in vivo physiological situation.
Mehrabi Boshrabadi et al. (2006) investigated the technological gap of wheat cultivation in five regions of Kerman province using stochastic frontier analysis and Translog production function.
Compared to other actuator types [15], the superiority of the membrane actuator made of an elastic polymer, i.e., Polydimethylsiloxane (PDMS), makes it suitable for cell cultivation because there is no gap in the actuator layer.
They have encouraged the cultivation of thirsty, soil-depleting cotton in the area: the GAP region is now a bigger producer of the white fluff than Turkey's traditional cotton-growing area around Adana.
In our paper this research gap is addressed for the sector of ornamental plant cultivation, by examining the network activities depending on the type of innovation, and the factors influencing the networking decisions.
They also reveal gaps in knowledge gained through practical experience about cultivation and using the nutrient value of manures.
Cultivation of banana by the early farmers in tropical rainforest gaps (Groube, 1989) could provide a favourable environment for intra- and interspecific hybridizations and growth of hybrid volunteer seedlings, which were subject to human selection.
We aimed at bridging this gap by contributing to the scientific discussion on the uncertainties of the cultivation of genetically modified (GM) crops in the region.
