Efforts to develop vaccines against these pathogens have been prevented by the difficulty of cultivation of parasites in the laboratory, the complexity of their multicellular organization and many species have been developing impressive antigenic variability.
Here we report the development of a diagnostic DNA microarray for the high-throughput identification of 39 pathogenic bacteria selected based on their high prevalence rate and/or difficulty of cultivation.
Owing to the inherent difficulty of cultivation of T. thermosaccharolyticum DSM 571, it is difficult to obtain a sufficient amount of cells for large-scale enzyme production.
Many medicinal plants, however, are still harvested in the wild due to technical difficulties of cultivation, as well as for economic reasons.
Persoonia levis is rarely seen in cultivation, mainly because of difficulties in propagation; seed germination is unpredictable, and cuttings have been nearly impossible to strike.
Persoonia lanceolata is rarely seen in cultivation, mainly due to difficulties in propagation; seed germination is unpredictable, and cuttings have proven difficult to strike.
Although marine algae and fungi are used in commercial production, the difficulties in cultivation and adaptation of marine microorganisms have changed the situation from conventional marine sources into nonmarine microorganisms.
Despite their ubiquitousness and apparent significance, our understanding of these microbial communities remains very limited, largely owing to their inherent complexity and the difficulty in laboratory cultivation of most of the microbes.
Microbial interactions in natural microbiota are, in many cases, crucial for the sustenance of the communities, but the precise nature of these interactions remain largely unknown because of the inherent complexity and difficulties in laboratory cultivation.
In 1994 the General Agreement on Tariffs and Trade limited subsidies, and French rice cultivation, still fairly uncompetitive, experienced difficulties once again.
