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Furthermore, the exact microbial concentration cannot be determined using only cultivation dependent methods since microbes may be viable but non-cultivable and culturing usually leads to underestimation of microbial diversity and concentration [22 26].
The hypothesis that male urethral microbiomes could impact STI has been tested using primarily cultivation dependent microbial identification methods [11], [12], [13], [14], [15], [16], [17].
Such survey should be accomplished through both molecular and cultivation dependent techniques that, respectively, allow (i) the correlation of the environmental parameters with the structure of the whole microbial communities and (ii) the enrichment, identification, and characterization of degrading microbes for traits of interest like the production of biosurfactant.
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This fungal community was studied based on cultivation-dependent method and therefore it is expected that twigs/knots harbor a much larger diversity.
His present research integrates high-throughput sequencing and cultivation-dependent approaches to study the evolution, ecology, and physiology of soil-dwelling Streptomyces.
We studied, under two different plant compositions, the short-term effects of glyphosate on rhizosphere soil microbial communities through the utilization of cultivation-dependent and -independent techniques.
This method also includes microorganisms that were not accessible with the cultivation-dependent efficiency analyses.
Efficacies of various dosages were verified in a double evaluation that combined cultivation-dependent techniques and differential staining microscopy.
Therefore, metagenomic analyses facilitate the discovery of novel genes in biological communities by overcoming the limitations of cultivation-dependent approaches (Guazzaroni et al. 2015; Leis et al. 2013).
Treatment efficiencies were assessed with cultivation-dependent methods in combination with a novel textile imprint method coupled with confocal laser scanning microscopy.
This cultivation-dependent approach exhibited the presence of novel bacterial isolates such as L. bovis sp. nov. and M. suwonense sp. nov. in organic formulations and PG ingredients, respectively (Anandham et al. 2011a, b).
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