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Both container types used were made of high-density polyethylene, cylindrical in shape, open-bottomed, with a diameter of 5 cm, two kinds of vertical ribs on the inside wall showing a cultivation density of 318 seedlings/m2.
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One of the most obvious effects is nutrient enhancement through mussel excretion at low cultivation densities, which promotes primary production particularly during the N-limitation period in summer.
After 24 h, 48 h, and 72 h cultivation, cell densities were measured with MTT [3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide] colorimetric assay.
A high density cultivation technique and outdoor cultivation have been used for F. solaris [ 8, 10].
Pure photoautotrophic production was possible using two media types, with recombinant protein accumulation in all cultivations correlating to culture cell density.
Proteolysis may occur either during vesicular transport of recombinant protein by secretory pathway-resident proteases (Werten and de Wolf 2005; Ni et al. 2008) or in the extracellular space by proteases being secreted, cell wall-associated (Kang et al. 2000) or released into the culture medium as a result of cell disruption during high cell density cultivation (Sinha et al. 2005).
However, making this proposal financially feasible would require industrial scale production of DHA through high density cultivation of microphytes.
During 100 h batch cultivation, the optical density of LEK513 reached 60, while LEK122 only grew to 7.5.
In high cell density cultivation processes the productivity is frequently constrained by the bioreactor maximum oxygen transfer capacity.
By contrast, growth of Escherichia coli was severely compromised under these high cell density cultivation conditions on crude glycerol.
Performance of the HRI for the high density cultivation of plant cells (27g dry weight/L) and insect cells (6×106 cells/mL) are presented.
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