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Based on relevant literature and validated seasonal cultivation data from the Algae Testbed Public-Private-Partnership (ATP3) project, as well as established data and methods for urban resource availability assessment and transport calculations, the overall energy performance of the decentralized system is estimated.
Glucose was not detected in the culture medium during cultivation (data not shown).
A plot of the cultivation data can be seen in Figure 3.
Within the BT- and BT-like category, Dian1-CMS hybrids are steadily replacing BT-CMS hybrids; the former now comprise ~90% of cultivation (data not shown).
As the NGAM had an obvious effect on the specific growth rate, the NGAM in the iRY1243 was firstly calculated using P. pastoris G1HL chemostat cultivation data without by-product formation.
Moreover, MnP was overproduced 3- to 4-fold in wheat bran (data not shown) and a 40% increase in compost was observed in transformants MnP1-1 and MnP1-2 relatove to A15 in a small scale cultivation (data not shown).
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FFAs droplets which were clearly seen in growth media and were attached to the fungal filaments immediately after mixing (Fig. 3) were not detected after 24 h of co-cultivation (data not shown).
For the following experiments discs containing 30% (w/w) sodium carbonate were used, because previous growth experiments with E. coli demonstrated that these discs have the most suitable release kinetics for the cultivations (data not shown).
D. sessilis was among the species treated, but as there was not yet any experimental data on cultivation, information was restricted to its aesthetic qualities and the soil in which it naturally occurs.
Fig. 2 Time courses of P. putida KT2440 pSynPro8oT_rhlAB bioreactor cultivation measurement data compared to modeled data.
From the cultivation process data, the maximum specific growth rate was estimated for each single substrate experiment and for the double substrate culture.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com