Sentence examples for cultivation cells from inspiring English sources

For CO2 enriched cultivation, cells were cultivated at 50 μmol photons m−2 s−1 and 1 or 2% CO2 was supplied with a flow rate at 80 mL min−1.

To prepare seed cultures for on-chip cultivation, cells expressing mCherry, GFP and EYFP were cultivated overnight in LB media containing 0.4% arabinose and cells labeled with CFP in LB media containing 0.2 mM IPTG.

After cultivation, cells were lyophilized for polymer extraction.

Following additional 3 h cultivation, cells were harvested by centrifugation at 10000×g for 15 min.

For further cultivation, cells were kept at 37 °C in 5% CO2 humidified atmosphere.

After 24 and 72 h of cultivation, cells were harvested, washed and kept at −80 °C until further lyophilization.

After cultivation, cell pellets were harvested and washed twice with 20 mM Tris HCl buffer (pH 8.0).

During cultivation, cell growth was measured spectrophotometrically at the wavelength of 620 nm (OD620 nm).

Furthermore, after 72 h cultivation, cell viabilities were assessed using Live/Dead Cell Staining Kits (Invitrogen) according to the manufacturer's instructions.

After 24 h, 48 h, and 72 h cultivation, cell densities were measured with MTT [3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide] colorimetric assay.

The cell growth and production of amylase by Bacillus sp. is reported to be dependent on the strain, composition, and concentration of media, methods of cultivation, cell growth, nutrient requirements, pH, temperature, time of incubation, and thermostability [ 22, 23].