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At K = 6, the same ORSC subpopulation groups were observed as when the data were analyzed without the O. sativa samples, but the cultivated samples allowed us to identify wild populations that clustered with specific O. sativa subpopulations (Additional file 3: Figure S2A, B).
Wild laurel presented lower GI50 values than cultivated samples but also higher toxicity against nontumor liver primary cells (PLP2; 114 μg/mL).
According to those results, cultivated samples showed higher concentrations of flavonols (e.g., quercetin and kaempferol derivatives) and flavones (e.g., luteolin and apigenin derivatives).
As it can be concluded, cultivated samples had higher contents in total phenolics, especially due to their higher contents in flavonols, since the amounts in flavan-3-ols were similar for both origins.
The same pattern was observed in both the P&I cultivated samples analyzed by Liu and Burke [ 46] and in the set of inbred lines analyzed by Kolkman et al. [ 47].
The patterns of pairwise LD differed greatly between the wild sunflowers and cultivated samples analyzed here: in the former group, the strong linkage disequilibrium was evidenced within distances <200 bp [ 46], whereas in the second group it was noticeable at least up to 700 bp.
Similar(19)
While Markello conducts further analysis of her million-SNP array, the geneticist Marjan Huizing and her colleagues are cultivating samples of Stiers's cells to look for fibulin directly, as well as for other relevant proteins like those involved in the maintenance of tight junction cells.
This observation is important for the bacteriologist when cultivating samples from cases of keratitis, and may be related to the intracellular growth of L. monocytogenes.
For one recent work, I cultivated bacterial samples from a hundred female friends and acquaintances, creating a piquant smell that permeated the gallery.
Sulfate-reducing bacterial consortia were cultivated from samples in a modified Postgate B medium (Postgate 1979) that contained potassium dihydrogen phosphate, 0.5 g/L; ammonium chloride, 1 g/L; calcium sulfate, 1 g/L; magnesium sulfate 7-hydrate, 2 g/L; yeast extract, 1 g/L; ascorbic acid, 0.1 g/L; thioglycolic acid, 0.1 g/L; and iron II) sulfate 7-hydrate, 0.5 g/L.
In addition, acetic-acid-producing microbes and sulfate-reducing microbes were cultivated from sampled environments containing FGE and water.
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