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A comprehensive reform was introduced in 1965 with three main objectives: to make the agricultural workers owners of the land they had cultivated previously, to increase agricultural and livestock production, and to facilitate social mobility and peasant participation in political life.
In this review we focus on new applications that have successfully cultivated previously uncultivated bacteria.
Populations were sampled at five sites in each of the following habitats: (1) gaps in tropical deciduous forest; (2) fallow corn fields cultivated previously under seasonal regime; (3) corn fields under seasonal agriculture; (4) corn fields under irrigated agriculture.
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Renewed efforts to cultivate previously "uncultivable" microbes will be important to the overall understanding of gut ecology.
Given that many appear capable of growth only at low substrate concentrations, the use of low nutrient conditions such as C-free minimal medium with agar has been demonstrated to cultivate previously uncultured microbes [ 13].
Bovine mammary epithelial cells (MAC-T) were cultivated as previously described [13].
Neonatal ventricular cardiomyocytes were isolated from 0 1 day old Wistar rats and cultivated as previously described [11].
D9 mouse ES cells, HR9 mouse extraembryonic endodermal cells and mouse trophoblast stem cells were cultivated as previously described [3], [40], [41].
Plants were cultivated as previously described [ 4].
P. falciparum parasites were cultivated as previously described [ 56].
Schizosaccharomyces japonicus cells were cultivated as previously described (Furuya and Niki 2009).
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CEO of Professional Science Editing for Scientists @ prosciediting.com