By ignoring many non-genetic sources of variation, and simply averaging over biological and technical replicates to obtain a cultivar value, cultivar selection may not be optimal and any planned subsequent genetic studies such as quantitative trait loci (QTL) mapping may be compromised.
Most high-throughput saccharification studies published to date have simply averaged over biological and technical replicates to obtain a cultivar value and have disregarded the impact of other potential sources of non-genetic variation such as time and date of analysis and position of samples within incubators.
The average value of the two-cultivar specific dependence ratios was used to estimate a cultivar independent value.
Similarly, in control plants of Pegaso cultivar, the value of RWC was measured as 95%.
For both cultivars, Ψpd values of irrigated plants, during the dry season, ranged from -0.11 to -0.38 MPa, demonstrating the absence of drought stress.
In support of this, [ 24] reported a range of grain (1,3 1,4 -β-glucan 1,3 1,4 -β-glucan 1,3 1,4 -β-glucan of barley contentss, while values of up to 13.4have been reported for wild barley (Hordeum spontaneum) [ 25].
Hippeastrum hybrids and cultivars are valued for their large ornamental flowers, particularly for indoor cultivation during the northern hemisphere winter.
In the irrigated condition, the I59 cultivar showed highest values of total RBCS1 expression, while RBCS1 expression in the Rubi, Icatú and Obatã cultivars was lower and more similar (Table 4).
In that analysis, Augusto and Eurosis resulted among the most tolerant cultivars, with RWC values > 60 % after 48 h of treatment, whereas Loto and Vialone Nano resulted the most sensitive cultivars, with RWC values < 15 % after 48 h of treatment.
In all cultivars, T 2eff values were found to be rather constant until October, thereafter they increased.
In Cluster 3, which included the F1 hybrid cultivars, high HZ values were found in the large regions of chromosomes 6 (8.7–30.0 Mb), 9 (4.8–58.9 Mb), and 11 (7.7–49.3 Mb) and in small segmental regions on all chromosomes.
