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Here, we evaluate the PD-L1 expression on 31 CTC-containing samples, obtained from 22 patients with metastatic NSCLC who were scheduled to receive or were receiving PD-1 or PD-L1 inhibitors, including 11 metastatic NSCLC patients scheduled to receive the anti-PD-1 treatment pembrolizumab (one patient ended up receiving erlotinib) (Fig. 1A, Table 1).
The slide also contains sample startups in each category.
In order to optimize the operation parameters for CTC purification by the Thermoresponsive NanoVelcro system, the first-type artificial CTC sample containing EpCAM-positive H1975 NSCLC cells (200 cells mL 1) and freshly purified human WBCs (5 × 10 WBCs mL 1) in a RMPI medium was prepared.
For instance, a TP53 subclone was only detected in cfDNA and CTCs in sample MM_2017 (Fig. 5a, c and Fig. 6a, c).
Five fields with the greatest number of CK19-positive CTCs or CD44/CK19 double positive CTCs were chosen to quantify the number of CTCs in the sample.
MM_2205 CTC sample used 0.7 power due to lower coverage.
ERCC1 levels were determined from average expression on individual CTCs in each sample.
The Mag-Gradient Chip can isolate the 3 ml of heterogeneous CTCs sample in 1 h with high isolating yield.
Table 2 contains the sample order used.
Samples (1 µl) were injected (using a CTC auto sampler) into the FID with a split ratio at 1 10.
The mean CTC number at the time of highest CTC value was 64 (SEM ± 30.6) compared to 2.4 (SEM ± 0.5) for the remaining CTC positive samples (P = 0.0001).
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