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Unlike BRAF genotypes, KIT genotypes were poorly matched between primary tumours, metastases, and CTC (Table 3).
We did not observe a better prognostic discrimination with the cutoff of one CTC (Table 1).
CTC prognostic value was observed only in metastatic IBC patients, with a threshold of one CTC (Table 3).
BRAF exon 15 was amplified from single CTC from 9 out of 11 melanoma patients and BRAF exon 15 sequences were obtained from 14 individual CTC (Table 3).
Although the majority of assays use EpCAM as detection marker, different markers are currently used to detect and enrich CTC (Table 2).
Similar(55)
We tested the robustness of this assay by evaluating FISH on samples with as few as 2 CTCs and many as 159 CTCs (Table 1).
In order to assess the reproducibility of our method to quantify HER2 expression on CTCs, 3 independent readers quantified the HER2 Intensity CellSearch® of 30 CTCs (Table S1).
As an example, some available technologies detect only EpCAM+ CTCs (Table 1).
There was significant correlation between the outcome of the treatment and the presence of CTCs (Table 1).
The correlation remained significant when the cut-offs for CTC detection with the CellSearch were set at ⩾1 and ⩾2 CTCs (Table 2).
There was no difference in terms of the main clinico-pathological characteristics in patients with detectable and undetectable CK-19mRNA-positive CTCs (Table 1).
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