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In 1556 (78.9%) of the 1972 samples analyzed for the presence of CTC before CHT using CSS, no CTC were detected, while 416 (21.1%) were positive for CTC (median 1 CTC, range 1 827 CTC).
In the majority of the 247 (20.6%) positive samples (median 1 CTC, range 1 256 CTC) only 1 CTC was detected (n = 148, 12.4%).
889 (83.4%) of the 1066 samples investigated for the presence of CTC after CHT using MICC were negative for CTC and CTC were found in the remaining 177 (16.6%) samples (median 1 CTC, range 1 23 CTC).
F* Assay failed by Quest CellSearch™ Our method had a significantly higher CTC detection rate than the CellSearch™ system (p < 0.01) and a wider dynamic CTC range (1 to 571 vs. 1 to 270).
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Single CTC PCR occasionally failed; the success rate of PCR amplification from single CTC ranged from 20%to100%0%.
Kurihara et al (2008) reported on 26 patients, 11 of whom had CTCs (range 1 105, mean CTC/7.5 ml 17, median, 5) with a mean and median CTC number for all 26 patients of 7 and 0, respectively.
The HER2 CEP17 ratios in the gene-amplified CTCs ranged from 2.0 to 18.3.
The number of CTCs and M30-positive CTCs ranged from 1 to 141 (median 2) and 0 to 67 (median 2) cells, respectively.
When a comparison was made between CTCs and prostate cancer cell line, the values of Young's moduli obtained for CTCs (ranged from 0.23 to 1.1 kPa) were similar to that of PC-3, thus inferring they are metastatic.
In a recent study, using a quantifiable, dual-colorimetric RNA in situ hybridization assay for epithelial and mesenchymal transcripts, Yu et al. [ 5] defined five categories of CTCs ranging from exclusively epithelial (E) to intermediate (E > M, E = M, M > E) and exclusively mesenchymal (M).
ER+ patients had the highest CTC counts (range: 0 30, median:8), while lower CTC counts were seen in HER2+ patients (range: 0 7, median:1) and in triple negative patients (range: 0 6, median:1) (Figure 5B).
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