Moreover, in our already published protocol without immunoselection we are using 20 mL of peripheral blood for CTC isolation in the adjuvant setting [ 6, 32], whereas in this case we are using cDNAs from EpCAM-positive CTC isolated from 2 × 5 mL peripheral blood.
ERCC1 mRNA levels were measured in selected CTC isolated upfront to cytotoxic chemotherapy.
Pancreatic CTC isolated from blood samples using FACS-based negative depletion, express a cell motility gene signature.
Pancreatic CTC isolated from blood samples using a FACS-based negative depletion method, express a cell motility gene signature.
From 6 single CTC isolated from one patient, 1 CTC had TP53 R110 delC, 1 CTC showed the TP53 R110 delG mutation, and the remaining 4 single CTCs showed the wild type p53 sequence; a pool of 14 CTCs isolated from the same patient also showed TP53 R110 delC mutation.
Indeed, we found that CTC isolated from metastatic bladder, breast, and skin cancer patients were also lipid rich (data not shown).
During the present study where the aim was to optimise immunofluorescence methods for the selective detection of MCT1 and 4 in CTC isolated from cancer patient blood samples, F-for-P was adopted.
A novel procedure is presented for the fit-for-purpose evaluation of the reproducibility of an IHC method for the determination of AR receptor expression levels in CTC isolated from patients as a biomarker assay utilising a modification of ISR and BCTI for statistical analysis of results.
Assuming that metastasis requires a dissemination of tumor stem cells or tumor cells showing EMT, we studied the expression of (1) the EMT markers Akt2, phosphoinositide kinase-3α (PI3K-α) and the transcription factor TWIST1 and (2) the stem cell marker ALDH1 in CTC isolated from 502 patients with primary breast cancer.
We next assessed whether CTCs isolated from preserved blood contain intact RNA compatible with tumor molecular profiling.
Moreover, we among others have shown recently the feasibility of performing targeted sequencing on single cells of CTCs or WES on CTCs isolated by multi-color flow cytometry3,10.
