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Delta Ct (dCt) values were calculated by subtracting the 18S rRNA Ct value from the target Ct value.
The ∆ CT value was determined by subtracting the CT value of the endogenous control from the experimental CT value.
First, a CT value is acquired from the CT image.
ΔCT is difference in CT value of the target gene from the CT value of the reference gene (16S rRNA).
The ΔCT value was determined by subtracting the CT value of the endogenous control from the experimental CT value.
The Ct of the target gene is normalised to the GAPDH PCR Ct value by subtracting the GAPDH Ct value from the target Ct value.
According to the comparative CT method, the CT value was determined by subtracting the H2az CT value for each sample from the CT value for each gene.
When a = 1000, the CT value is Hounsfield units (Hu).
Final results were measured as Ct value.
The threshold cycle (Ct) value for RelA was normalized to the Ct value of GAPDH.
This then gave a delta CT (ΔCT) value for each miRNA (miRNA CT value – RNU44 CT value).
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