Suggestions(2)
Exact(14)
Samples were then converted to a fold change ratio described using standard Δ CT formula where ΔCT = CT target – CT average endogenous controls.
To obtain linear Relative Quantification (RQ) values, relative expression was assessed as (40-dCT), as previously described, whereby dCT (or deltaCT) was calculated as (average target CT) – (average GUSB CT) from all eligible measurements [ 13].
To obtain linear Relative Quantification (RQ) values, relative expression was assessed as (40-dCT), as previously described (Koutras, Kalogeras et al. 2008), whereby dCT (or deltaCT) was calculated as (average target CT) – (average GUSB CT) from all eligible measurements.
To obtain linear Relative Quantification (RQ) values, relative expression was assessed as (40-dCT), as previously described, whereby dCT (or deltaCT) was calculated as (average target CT) – (average endogenous control CT) from all eligible measurements [ 7].
Heterotrophic nitrification rates were very low in NT soil, and ranged from 0.02 0.11 mg N·kg− 1 d− 1, which were significantly lower than those in CT (average rate of 0.80 mg N·kg− 1 d− 1).
Ct, average.
Similar(45)
PCRs were run in triplicates for each sample and Ct averages were obtained.
CDC2 cycle threshold values (Ct) averaged 18.5 + 0.5 (Standard Deviation), which is the experimental error range of the RT-qPCR cycler device.
The number of participants was rather low in most studies and ranged from 13 to 72 in RCTs (average 37), from 8 to 74 in CTs (average 31).
CT-time averaged, CT cross-sectionally averaged, and outflow methods gave average values of 0.0789, 0.0818, and 0.140 mm/s, respectively.
The average ΔCt of each group was calculated by the following formula: ΔCt = average CAV1 gene Ct-average of HK (housekeeping) gene' Ct.
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