Sentence examples for ct assay from inspiring English sources

The 2−ΔΔ Ct assay does this by normalizing the amount of a variable-copy target gene, against an endogenous single copy gene.

Calibrated with a reference genome 'spike', and including primers for a single copy housekeeping gene for normalization, the 2− ΔΔ Ct assay thus appears valid as a gene counting method for var2csa genes.

The 2−ΔΔ Ct assay results, showing the proportion of samples containing parasites with multiple var2csa-type genes are presented in Table 2. Parasites derived from the placentae were more likely to contain clones which have multiple var2csa-type genes, although this inequality was not statistically significant.

Main outcome measure Diagnosis of chlamydia infection with samples analysed using the Aptima Combo-2 assay; positive results confirmed with the Aptima CT assay.

Of the six indeterminate vulvovaginal swab results, three were positive with the Aptima Combo-2 assay but unconfirmed by the Aptima CT assay.

In the BioStation CT assay, the spheroids moved at an average of 7.19 pixels per hour during the observation period in the drug-free well (red line); however, a 68% reduction in spheroid migration velocity was seen with the addition of paclitaxel (Fig. 4c and Suppl. Video S2c), but only a 6% reduction with trastuzumab (Fig. 4c and Suppl. Video S2b).

A series of long-term monitoring were performed at various time points (4w, 12w, 26w and 52w) after implantation using numerous investigations such as micro-CT assay, histomorphometric analysis, local micro-environment testing and biochemical analysis of serum and urine.

To compare matched samples, a fold-change analysis was used (2(case∆Ct - control∆Ct), ∆Ct = Raw endogenous control Ct – Raw assay Ct) and to identify the most significantly deregulated miRNAs a 3-fold change cut-off was applied (selection of fold-change (FC) cut-off explained in results).

To determine the efficiency of the duplex real-time PCR assay, Ct values obtained from a series of template DNA dilutions were graphed on the y axis versus the log of the dilution on the x axis.

LT and CT were assayed by the ganglioside monosialic acid enzyme linked immunosorbent assay (GM1-ELISA) [ 29].

The Presto CT-NG assay and the Lightmix Kit 480 HT CT/NG detected N gonorrhoeae equally, but for a definitive validation more samples are needed.