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Presumed functional domains within the teleost control region, such as conserved sequence blocks (CSB) in the central conserved region, CSB-II, and CSB-III (as identified in Lee et al. [ 46]), are all highly conserved across Fundulus species.
Thus, it appears that the increased binding of CSB to map2 promoter paralleled the recruitment of BAF, thereby implying a role for CSB in BAF recruitment.
Importantly, CSB binding to oxidative damage was reduced upon transcription inhibition which suggests a role of CSB in transcription-associated repair of oxidative lesions.
To test the role of CSB in vivo, we measured intergenerational and somatic expansion of CAG tracts in HD mice lacking CSB, OGG1, or both.
The VHS domain of UVSSA is suggested to be also involved in the interaction between UVSSA and CSB, since a UVSS-A patient missense mutation (Cys32Arg) within the VHS domain disrupts the interaction with CSB in response to UV.
Consistent with reduced MAP2 expression in CSB-depleted neural cells, tandem affinity purification and chromatin immunoprecipitation studies revealed a potential role for CSB in the assembly of transcription complex on MAP2 promoter.
Similarly, silencing CSB led to increased apoptosis following exposure to UV light (data not shown), consistent with a role for CSB in determining the sensitivity of cells to apoptosis induced by both of these agents [ 6, 16- 18, 50, 59, 60].
All measurements were made by the same author (CSB) in a blinded manner using the eNTEGRA system.
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Moreover, a deeper comprehension of the statistical properties of the CSBs in different noise and clutter distributions, e.g., the widely known compound-Gaussian distributions, has to be developed.
As concerning the estimation accuracy and the detection performance, we found that the FB slightly outperforms the CSBs in the white noise scenario, whereas the four CSBs outperform the classical FB in the spatially correlated noise scenario.
These structures could correspond to CsBs in the larch.
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