Hence, RIBER complements DIBER for analyses of crystal content in crystallization trials of protein nucleic acid complexes.
In crystallization trials, SopDΔN produced better crystals than either full-length SopD or trypsin-digested SopDΔN.
Crystallization trials with 1 yielded small crystals that we were unable to obtain with the apo protein or with ADP.
Crystallization trials revealed that both 2′-azido modified RNAs crystallized, providing crystals diffracting to atomic resolution (Supporting Table 2).
Crystals of rfOmpC were obtained in preliminary crystallization trials.
This approach is usually applied when crystals of inadequate quality or crystalline precipitate are obtained from initial crystallization trials.
Seed stocks made from crystals of the mutant enzymes were used in subsequent rounds of crystallization trials.
The sample was concentrated for crystallization trials.
Crystallization trials were carried out using the hanging-drop vapour-diffusion method35.
Initial crystallization trials were performed at 20 °C using 6.5 mg/mL protein by sitting-drop vapor-diffusion in 96-well MRC-crystallization plates (Molecular Dimensions).
Crystallization trials were set up in 96-well glass Laminex sandwich plates (Molecular Dimensions) using an LCP Gryphon (Art Robbins Instruments).
