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The crystals grown from crystallization solutions containing PEG were cryoprotected using the corresponding crystallization solution containing 25% (v/v) glycerol in place of water.
The third major difference between the in vivo and in vitro crystallization conditions might be overcome using the appropriate crystallization solutions (Dorozhkin et al. 2004).
Impurities in the molecules or in the crystallization solutions are often inimical to crystallization.
In practice, favorable conditions are identified by screening; a very large batch of the molecules is prepared, and a wide variety of crystallization solutions are tested.
Hanging drops were formed by mixing equal volumes of protein and crystallization solutions.
It is also interesting that while both the BAU-bound and 5-FU structures were crystallized under very similar conditions, only the former was found to have bound phosphate, presumably chelated from trace contamination in the purification or crystallization solutions.
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Ternary complexes of EGS, NADP+ and the EGS inhibitor EMDF were obtained by soaking EGS/NADP+ crystals in crystallization solution supplemented with 5 10 mM EMDF.
Before diffraction experiments, crystals were soaked in crystallization solution containing 30% glycerol for cryoprotection.
Before diffraction experiments, crystals were soaked in crystallization solution containing 10%-30 10%-30rol for cryoprotection.
For the structural investigation of the complex of furin with I2, crystals were soaked in crystallization solution supplemented with 3 mM of I2.
Crystals were mounted directly from the crystallization solution and flash-cooled in liquid nitrogen.
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