Sentence examples for crystallization screening from inspiring English sources
Our work demonstrates the effectiveness of using Crystal Formers as an autonomous crystallization screening tool for producing diffraction-quality crystals and can be the stepping stone for future structure determination.
However, attempts to obtain crystals for this complex were not successful after an extensive crystallization screening.
The initial crystallization screening trials of the purified PLldR were performed manually by the method of hanging-drop vapour diffusion in 24-well plates at two different temperatures (287 and 297 K).
When a crystallization screening experiment yields no hits, it is hard to know if the cause was an unsuitable temperature or unsuitable solution conditions.
Numerous vapor diffusion experiments were initially set up at room temperature using commercially available crystallization screening kits, as well as utilizing five different constructs of LidL (full length and various truncations) at concentrations typical of most vapor diffusion experiments (<20 mg/mL).
hAFP was crystallized by the hanging-drop vapor-diffusion method at 298 K. Initial crystallization screening was performed using crystal screens 1 and 2 from Hampton Research.
The other constructs which ultimately produced a two domain version of the structures RrgBD2 D3, (containing residues 141 592 of full length RrgB) were produced with identical crystallization screens in crystal condition 30% PEG-6000 0.1 M Citrate pH 5.0 at 20°C.
Several crystals were obtained from crystallization screens.
Random Microseed Matrix-Screening (rMMS), where seed crystals are added automatically to random crystallization screens, is a significant recent breakthrough in protein crystallization [110].
Our results suggest that the addition of heterogeneous nucleating agents is a simple method for increasing the chances of crystal formation when using sparse matrix crystallization screens.
We compared several heterogeneous nucleating agents and showed that the addition of some of these provides a simple method to increase the chances of crystal formation when using sparse matrix crystallization screens.
