Sentence examples for crystallisation plate from inspiring English sources

For the hanging drop method, the protein drop is suspended above the well solution from a seal and makes no contact with the crystallisation plate.

While previous studies have examined the effects of protein construct, protein purity, or crystallisation condition ingredients on protein crystallisation, few have examined the effect of the crystallisation plate.

For the sitting drop method, the protein drop sits directly on a surface of the crystallisation plate above the crystallisation condition.

Twelve 85 µL replicates of a 10% w/v PEG 4000 solution were pipetted into the same 10 types of sitting drop plates and one hanging drop crystallisation plate used above, and sealed and incubated as described above.

The MPL has available a fully automated nanodrop crystallisation system, consisting of a Hamilton Star liquid handler for plate preparation, a Cartesian nanodrop robot for preparation of drops as small as 100 nl, automatic plate sealers and Rhombix imagers at both 4 and 20 °C, which can record 96 images from a crystallisation plate in 10 min.

The crystallisation condition (85 µl) was placed in the reservoirs of the crystallisation plates using a Biomek® 2000 Laboratory Automation workstation (Beckman Coulter, California, USA).

Ten commercially available 96-well sitting-drop crystallisation plates (Table 1) were selected on the basis of diversity of material and sitting-drop well geometry.

Potential crystals are screened in crystallisation plates using the PX scanner system, an in-house X-ray system.

To investigate the molecular basis for HpHb binding by TbHpHbR, crystallisation plates were set up for HpSPHb, TbHpHbR and a complex containing TbHpHbR bound to HpSPHb.

Crystallisation conditions were found using our in house high-throughput crystallisation platform [20], mixing 100 nL SeMet ZapC solution at 5 mg/mL with 100 nL of 1920 different crystallisation reagents in MRC vapour diffusion sitting drop crystallisation plates.

Many soluble protein structural genomics groups have the necessary technology to clone, express and purify many protein targets in parallel, with clones being generated on a 96-well plate scale and crystallisation trials being completed at the rate of 100 crystallisation plates per day [ 57,58].