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Crystallization conditions for the purified proteins were screened with Crystal Screen I and II crystallization screening reagents (Hampton Research, Aliso Viejo, CA, USA) by sitting-drop vapor diffusion in IntelliPlates (Art Robbins, Sunnyvale, CA, USA).
Initial crystallization trials were set up using Crystal Screen I (Hampton) and MembFac (Nextal).
Subsequently, the Wizard crystal screen was carried out to identify the initial crystallization conditions.
Crystals were screened by the sitting-drop vapor diffusion method (Jancarik et al., 1991) using sparse-matrix screen kits Crystal Screen I and II (Hampton Research), followed by optimizing the crystallization conditions through the variation of protein concentrations and pH.
Initial crystallization conditions were screened at 4°C and 20°C with Index and Crystal screen 1 and 2 (Hampton Research, Aliso Viejo, CA) and JBS classic (Jena Biosciences) in sitting drop setups using 96-well plates from MRC.
The blingy Swarovski D light bangle blinks the time on its crystal screen as if it were a DVD player in need of your attention ($1,500 in stainless steel, $1,700 in gold).
Similar(38)
hAFP was crystallized by the hanging-drop vapor-diffusion method at 298 K. Initial crystallization screening was performed using crystal screens 1 and 2 from Hampton Research.
Sapphire crystal screens: why Apple is interested in a gemstone.
Some very high-end smartphones currently use sapphire crystal screens.
Corning claims that Sapphire crystal screens would cost around 10 times as much as its Gorilla Glass.
It is unknown whether Apple has developed a way to make thinner, stronger sapphire crystal screens.
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