This novely invented multicolor tracing technique proved that descendants of stem cells constantly went through neutral competition that drived all crypts toward monoclonality in a few months (75% crypts monoclonal in 2 months and 100% in 6 months).
In addition, the proliferative zone in MTX-rats moved progressively upwards in the crypts toward the crypt-villus junction.
The proliferative zone in MTX-rats moved progressively upwards in the crypts toward the crypt-villus junction.
While the proliferative zone in MTX-rats moved progressively upwards in the crypts toward the crypt-villus junction, the proliferative zone of MTX-ARG rats was only mildly affected, showing a slight shift upwards within the crypts.
In the study, another Wnt target gene, the receptor tyrosine kinase EphB2 (ephrin type-B receptor 2), was used to sort intestinal cells as it is decreasingly expressed from the bottom of crypts toward the upper differentiated area.
Hearing the cigarettes' siren call, the matches crawl from their box like zombies from a crypt, sliding toward their inevitable doom.
The TA cells migrate upwards along the crypt wall toward the luminal surface to give rise to terminally-differentiated (TD) cells that either undergo apoptosis and/or are shed into the lumen and transported away [ 4].
Mutational analysis of mice heterzygous at the Dbl-1 locus showed that crypts drift toward monoclonality in the small intestine [17], [24].
The putative schema for crypt-villus axis involves newly proliferated enterocytes in the crypt base moving toward the villous apex, in which enterocytes mature and differentiate into tight monolayers during the migration process, and ultimately slough off at the 'extrusion zone' [ 14, 39, 40].
Depending on the duration of ischemia, this sloughing may continue toward the crypt, causing denuded villi [17], [18].
