Sentence examples for cryopreserved within from inspiring English sources

To investigate the fertilization ability, chromatin structure, and DNA integrity of spermatozoa after being cryopreserved within an empty zona pellucida (ZP).

Fresh samples were processed within 48 h from collection, while frozen samples were processed and cryopreserved within 24 h from collection.

In addition, PBMCs and SFMCs were cryopreserved within 2 hours of isolation and stored in liquid nitrogen in culture medium supplemented with 50% FBS and 10% dimethyl sulfoxide.

For tissue preparation, embryos were cryopreserved within 30% sucrose in 0.1 M PBS overnight at 4°C followed by overnight freezing in tissue freezing medium (Triangle Biomedical Sciences) at −80°C.

The present study has been made possible thanks to the availability of the corresponding patient fibroblast/lymphoblast cell lines and/or DNA samples, cryopreserved within the laboratory "Cell Line and DNA Biobank from Patients affected by Genetic Diseases".

If the tumor tissue was larger than 0.5 cm in diameter at the intra-operative frozen section, a representative piece containing more than 60% tumor cells was cryopreserved within five minutes and made available to the Stiftung Tumorbank Basel for molecular examination.

To evaluate angiogenesis, the rat aorta ring assay was performed for both fresh and cryopreserved AM within 7 days.

Samples were aliquoted anaerobically and cryopreserved (−80°C) within 24 hours.

For rosetting and flow cytometry experiments, RBCs were cryopreserved in glycerolyte within 24 h of collection and thawed by standard methods (Kinyanjui et al., 2004, Deans et al., 2006) the day before an experiment.

Peripheral blood mononuclear cells (PBMC) were processed and cryopreserved from whole blood within 8 hours of venipuncture as previously described [17].

To develop molecular tools increasing the sensitivity of breast cancer micrometastases detection within ovarian tissue cryopreserved for fertility preservation.