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In this report, we performed orthotopic ovarian transplantation using fresh and/or cryopreserved whole ovaries from non-pubescent mice and demonstrated the efficiency of these immature (defined as preserved before puberty) cryopreserved ovaries to restore both endocrine function and fertility even in immature recipients.
To evaluate the feasibility of contralateral autotransplantation of cryopreserved whole ovaries with microanastomosis of the ovarian vascular pedicle.
Recently, it was shown that eggs and sperm could be generated from cryopreserved whole testes of rainbow trout (Lee et al. 2013).
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Peripheral blood mononuclear cells (PBMC) were processed and cryopreserved from whole blood within 8 hours of venipuncture as previously described [17].
After a 30-min incubation at room temperature, we cryopreserved the whole pups in OCT medium, and cryosectioned sagittal sections (10 μm).
Similar to SSCs and Leydig cells, VASA positive cells were less susceptible to damage when they were cryopreserved as part of whole tissue.
The SEM images of CD4+ lymphocytes from both cryopreserved PBMC and fresh whole blood show dense microvilli structures on cell surfaces.
For obtaining SEM images of CD4+ T cells from heparinized normal donor whole blood, cryopreserved PBMC and lyophilized control cells, CD4+ lymphocytes were sorted using a FACSAria II sorter.
SEM images of the CD4+ T cells purified from cryopreserved PBMC, normal donor whole blood and lyophilized Cyto-Trol cells were obtained using sample preparation procedures described in the 'Materials and Methods'.
The 16% lower CD4 expression level measured for the lyophilized control cells compared to cryopreserved PBMC and fresh whole blood using both quantitative flow cytometry and mass cytometry, was previously hypothesized to be due to steric interference associated with anti- CD4 antibody binding to the smaller size lyophilized control cells.
However, multiple inoculations with subinfective doses of whole cell infected cryopreserved erythrocuytes followed by immediate antimalarial treatment conferred T cell mediated protection against infection in a challenge study [17].
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