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The granular cells cryopreserved under these conditions exhibited a cell viability of 82.7 ± 2.7% and a viable cell yield of 28.6 ± 2.2%.
Cells were cryopreserved under the following conditions: 10% v/v DMSO in the sample and stored in LN2 (blue); 10% v/v DMSO and10% w/v Ficoll 70 and stored in LN2 (purple); 10% v/v DMSO and stored in a −80 °C freezer (red); 10% v/v DMSO and 10% w/v Ficoll 70 and stored in a −80 °C freezer (green).
The HFC was cryopreserved under protection of 10 % DMSO (PanEko, Russia) and 20 % FBS on the nutritive medium base.
Plasmodium berghei ANKA parasites were also kindly donated by Dr. William Jarra and were cryopreserved under liquid nitrogen.
Some of the hESC lines have been banked (cryopreserved) under good laboratory practice procedures with minimal involvement of animal components [ 15- 17].
ELS cryopreserved under both conditions each showed very good propensity to return to normal cell replication as post-thaw culture extended beyond the first 24 h.
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We compared both CPA solutions with embedded MSCs within PRP-SF bioscaffolds non-cryopreserved, cryopreserved without CPA or cryopreserved with sucrose 7 days after thawing (Supplemental Fig. 2).
Non-cryopreserved and cryopreserved with the combination of DMSO 10% and Sucrose 0,2 M samples showed high viabilities, although lower than DMSO cryopreserved samples.
Peripheral blood mononuclear cells cryopreserved for 12 years under these conditions have remained viable and immunocompetent.
PBMC cells are washed with phosphate-buffered saline (PBS), counted under microscope and cryopreserved using Cell Freezing Medium (5%DMSO/111%HSA in PBS).
Efforts to improve these technologies provide indirect support for the idea that the brain, like any other organ, may be adequately cryopreserved by current methods or methods under development.
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