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This B. henselae strain was isolated from the blood of an AIDS patient with bacillary angiomatosis and was cryopreserved after only several passages on agar.
We initially studied cryopreserved PBMC only from 24 patients with sepsis and 12 hospital controls (Table 1), enroled in a previously reported longitudinal study of endothelial function in sepsis [ 37], that were representative of the entire cohort in terms of age, gender, ethnicity and disease severity.
Only cryopreserved cells were evaluated for T cell zeta-chain allowing the direct comparison of longitudinal sepsis and control samples within experiments.
The following procedures are banned: the use of fresh donor sperm (only cryopreserved after control of the donor for sexually transmitted diseases after 6 months); postmortem fecundation using the stored sperm of the husband after his death; cloning of human beings.
The limitations of the measured posaconazole levels in our analysis are that they took place retrospectively from excess material remaining after laboratory analyses which had been routinely cryopreserved, and that only on arbitrary time points after day seven (the point commonly known to be the time when a steady state of posaconazole levels plasma is reached) were possible.
As there are no commercially available cryopreserved (human) liver slices, only fresh (human) liver slices can be used for metabolite profiling studies.
Interstingly, while the antibody (4H84) recognizes the FHC functions in both cryopreserved and formalin fixed paraffin embedded sections, the antibody that recognizes the conformed HLA-G (MEM-G9) functions only on the cryopreserved sections.
Cryopreserved decellularized scaffolds (DC) and scaffolds that were decellularized after cryopreservation (CD) were compared to fresh valves (F), cryopreserved native valves (C), and decellularized only scaffolds (D).
59 Indeed, it has been proposed that success rates would increase if all, not only supernumerary, embryos were cryopreserved.
Currently, researchers using highly multiplexed gene analyses are limited to working with only the highest quality cryopreserved tissues.
Although senescence-associated β-galactosidase activity is used as a marker, it can only be applied to cryopreserved tissue.
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