Sentence examples for cryopreserved in a from inspiring English sources

The constructs were cryopreserved in a vitreous cryoprotectant (CPA) with a multi-step protocol.

The T cells were cryopreserved in a solution of 90% fetal calf serum and 10% dimethylsulfoxide (DMSO) at 1 × 108 cells/vial.

Cells were cryopreserved in a 1.8 ml solution of 50% IMDM, 40% FBS, 10% DMSO (0.2 µm filtered), and shipped on dry ice.

Brains were kept in PFA overnight at 4°C and subsequently cryopreserved in a 20 30% sucrose/0.1 M phosphate buffer solution until sectioning on a microtome apparatus (30 µm thickness sections, Microm Zeiss).

Prior to sectioning on the cryostat, brains were cryopreserved in a 30% sucrose solution in PBS.

Tissue pieces (120 to 500 mg) were cryopreserved in a similar manner.

The successful vitrification of blood vessel segments using marginal conditions of slow cooling and rewarming, provide essential information for the development of scale-up protocols that is necessary when clinically relevant size samples need to be cryopreserved in an essentially ice-free state.

These human islets were isolated and cryopreserved in an era when many of today's modern techniques were undeveloped or prohibitive in terms of cost.

The remaining cells were cryopreserved in cryopreservation medium composed of dimethylsulfoxide (Sigma Aldrich) (10% in FBS).

The first ovary removed was perfused with 30 ml of CPA over a 60 min period and the second ovary removed was perfused with 5 ml of CPA over a 10 min period, before both ovaries were cryopreserved in the same run of a Planer controlled rate freezer as described above.

A testicular tissue piece of 7.5mg cryopreserved in cryovial using 1.5M DMSO, an equilibration time of 30 min at 4 °C showed fewer morphological alterations than the other protocols tested.