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Thus, the cells were cryopreserved from 4th to 6th passages.
But many in the field will be waiting for the next steps: testing whether the resulting mouse pups are genetically normal, trying out the technique in other animals, and using other and less controversial types of cells—such as stem cells that can be extracted and cryopreserved from adult testicular tissue as the starting point.
Peripheral blood cells were isolated and cryopreserved from all children and their mothers and some fathers.
HPTEC and HRTC are successfully cryopreserved from both isolated cells and cell suspensions obtained after trypsinisation, maintaining a normal growth after defrosting.
Peripheral blood mononuclear cells (PBMC) were processed and cryopreserved from whole blood within 8 hours of venipuncture as previously described [17].
Infertile patients seeking ART are at higher risk to carry HPV in sperm (10% with respect to 0% of fertile controls) [14] and HPV has been demonstrated in about 6% of sperm samples cryopreserved from testicular cancer patients [15], who need ICSI to become father.
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DNA was extracted from cryopreserved isolates from the Swiss Tropical and Public Health Institute, Basel or the University of Bristol.
Hepatocyte suspension cultures were prepared from commercial cryopreserved hepatocytes from non-transplantable liver tissues purchased from Promocell (Vitaris AG, 6340 Baar, Switzerland).
We also applied the technology to determine chromosome copy number on 134 single-blastomeres derived from 26 cryopreserved embryos from six unrelated couples.
Clonal growth was obtained from fresh biopsy samples, from cryopreserved tissue, and from xenografts of the tissues in immune-suppressed mice.
Whenever available, TILs were assayed against autologous tumor cells (cryopreserved directly from tumor tissue or cultured from tumor tissue).
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