Exact(29)
And vitrification has enabled the successful cryopreservation of those parasitic species not able to be cryopreserved by traditional methods.
Most parasitic protozoa respond to cryopreservation stresses similarly to other single cell suspensions, but few species are currently routinely cryopreserved by protocols specifically designed for vitrification.
OMCS were cryopreserved by storage in freezing medium (Cell Banker 1®) at −80 °C.
In this study, apical meristems from adventitious buds of three lily (Lilium L). cultivars were successfully cryopreserved by droplet-vitrification.
Devitrification of the intracellular solution can cause significant damage during warming of cells cryopreserved by freezing or vitrification.
Our hypothesis is that the embryos of amphibian species at risk of extinction could be cryopreserved by vitrification, using methods which have proved successful with fish oocyte.
Similar(31)
Because of the advantage of keeping the CMMB on the sequenced C57BL/6J background for future widespread ease of use of the mutant mice, and the inherent difficulties in recovering C57BL/6J mice from cryopreserved sperm by in vitro fertilization (IVF), we applied intracytoplasmic sperm injection (ICSI) of cryopreserved sperm for the recovery of mutations.
Cell surface antigens were used to identify the cryopreserved ASCs by flow cytometry.
B-lymphoblastoid cell lines Capo-LC L) were established from the patient cryopreserved PBMCs by the incubation with EBV-containing supernatant from the B95.8 cell line (ATCC).
The obtained culture collections of microbial strains are kept either cryopreserved or by means of sequential transfers.
The responses measured in cryopreserved PBMC by the old protocol were clearly inferior to those measured when freshly isolated.
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