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They had been cryopreserved at the 2 8-cell stage using a three-step propanediol cryopreservation kit (Freeze kit 1; Vitrolife AB, Gothenburg, Sweden).
All supernumerary embryos were cryopreserved at the pronuclear or blastomere stage.
These spermatozoa were successfully cryopreserved at the recipient facility and, yielded post-thaw fertilization rates of 27.6 66.4%.
Regardless of the cryoprotectant used, lower rates of in vitro development were obtained when the embryos were cryopreserved at the morula stage, and higher rates achieved using embryos at blastocyst stages.
To compare outcomes for patients randomized to have all embryos cryopreserved at the blastocyst stage or at the bipronuclear stage with subsequent post-thaw culture to the blastocyst stage.
Excess pre-embryos were cryopreserved at the pronuclear stage and later transferred into monitored natural cycles on the day after ovulation.
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Moreover, hESC and hiPSCs could be cryopreserved at −80°C by the slow-freezing method.
The samples were allowed to clot, and serum was separated by centrifugation and cryopreserved at -40°C, until the time of cytokine and C-RP determination.
The serum specimens were cryopreserved at −80°C to further assay for the level of antibodies to the recombinant antigens rESAT6 or rCFP10 by solid-phase enzyme immunoassay [ 26].
These cells were cryopreserved at passage 3 by the manufacturer and were routinely cultured for at least 10 population doublings.
After thawing, only the cells cryopreserved at −80 °C with 10% Ficoll 70 provided a plating efficiency comparable to that of the LN2 storage control (Fig. 2).
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