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He said the problem was caused by the egg-shaped bone crushing tissue underneath it over time.
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Large bowel lysate was collected after crushing tissues in liquid nitrogen.
In this experimental bite wound model containing contaminated, crushed tissue, irrigation with various solutions of cefazolin plus penicillin G did not reduce quantitative bacterial counts more than 3.1 log total bacteria/gram tissue.
Total protein lysates were made by suspension of crushed tissue with lysis buffer (1% Igepal CA-630, 50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 10% glycerol) containing protease inhibitors and incubation at 4°C for 30 min. with constant agitation.
Ice-cold hypotonic buffer was added to crushed tissue in a 5 μL:1 mg ratio.
IGF-I levels measured in mammary gland tissue were measured by first crushing the tissue under liquid nitrogen using a metal pestle and mortar.
To distinguish rapidly between various samples, flavonoids were extracted from flowers by immersing (without crushing the tissue) in solvents (HPLC method, we investigation for a patent RO122836-B1).
The principle method of wounding is the crushing of tissue in the path of the bullet, and this is called the permanent wound cavity.
Crushing the tissue samples in liquid nitrogen created tumour powder.
After crushing the tissue was fixed with freshly prepared 1% formaldehyde in PBS for 12 min.
The spleens were cut with scissors and cell suspensions were prepared by crushing the tissue pieces between two glass slides in PBS.
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