Bones and spleens were crushed, cells resuspended and filtered to obtain a single-cell suspension that was analyzed by flow cytometry on a FACSCanto (BD Biosciences).
Additionally, cells of pathogenic fungi cells crushed with the cell wall and cell membrane fracturing.
In Homs, the central city that was shelled during most of February and early March by government forces intent on crushing insurgent cells there, activists reported by Skype that another stretch of shelling, which began last week, intensified on Monday, hitting an older neighborhood with historic mosques and churches.
Wounding of leaves leads to an almost immediate crushing of cells with a loss of their integrity and compartmentalization.
The pitted vessels and mechanical cavities owe their origin to the crushing of cell fibres (Fig. 4c f).
This plastic pre-buckling stage with axial crushing of cell walls correlates with the uniaxial compressive response of the bulk specimens tested.
We interpret this to mean that such brittle, porous materials as the glass foam (and perhaps highly porous, brittle, cryogenic ice) do not exhibit plastic deformation before failing completely by the crushing of cell walls.
The vacuolated tapetal zone cells enlarged, eventually crushing the sporogenous cells.
The big glass beads may be efficient for dispersing cells from clusters, and the small ones are dedicated to crush the cells.
The eggs were then crushed and cells were forced through the nylon mesh strainer with a cell scraper (BD Falcon), into a six well cell culture plate containing 3 ml of TNMFH culture medium [ 26] supplemented with 10% fetal bovine serum (HyClone , Inc.
