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In this experimental bite wound model containing contaminated, crushed tissue, irrigation with various solutions of cefazolin plus penicillin G did not reduce quantitative bacterial counts more than 3.1 log total bacteria/gram tissue.
Total protein lysates were made by suspension of crushed tissue with lysis buffer (1% Igepal CA-630, 50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 10% glycerol) containing protease inhibitors and incubation at 4°C for 30 min. with constant agitation.
Ice-cold hypotonic buffer was added to crushed tissue in a 5 μL:1 mg ratio.
Radical debridement of all non-viable and crushed tissue is essential to limb survival.
Total DNA was extracted from the crushed tissue using the cetyltrimethylammonium bromide (CTAB) method [ 58].
Crushed tissue was lysed in TNESV buffer and 50 μg of tissue protein lysate was immuonblotted as described previously [ 26].
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He said the problem was caused by the egg-shaped bone crushing tissue underneath it over time.
Large bowel lysate was collected after crushing tissues in liquid nitrogen.
Storm Leaves Long Trail Of Damage Along Gulf The remnants of Hurricane Ivan, greatly diminished but still dangerous, churned a path north, leaving felled trees, buildings crushed like tissue paper and hundreds of thousands without power in Alabama and Florida.
Wound edges were clamped with a hemostat for five seconds to create crushed, devitalized tissue within each wound.
Levels of miR-29 and miR-223 were normalized to U6 snRNA and relative fold change between control and crush tissue was determined using 2-ΔΔCt method.
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