The two or three most recently formed somites of 12 20 somites present in the chick embryos were crushed or removed by using a titanium needle (McCrone Microscopes, Westmount, IL).
Then the shell was crushed and removed, the snail tissue was equilibrated in 30% sucrose overnight, embedded in tissue freezing medium (TBS), and cryosectioned.
The best sign to look to indicate assigned sex is the Adam's Apple, though even this can be crushed and removed for transgender women.
More than a year of grueling work and crushing disappointment removed from the first pass he threw in Denver, nobody would want to hide the next one he throws.
Afterwards, the grains are dried and crushed to remove the germ.
The mesocarp and epicarp pieces of fruits were lyophilized overnight whilst the seeds were crushed to remove the endocarp.
Dried petals can be used to create decoration or crushed to remove the oils.
Lightly crush and remove the hard portion from the inside.
Check it after dark and crush and remove any snails or slugs hiding there.
