The strawberry sorbet tasted like red ripe fruit that had been simply crushed and frozen.
This unctuous nectar, called cidre de glace in French, is made from apples that are picked and crushed while frozen.
Others were minced or crushed after freezing in liquid nitrogen, and DNA was extracted by using an IsoQuick nucleic acid extraction kit (ORCA Research, Bothell, WA, USA).
The cells and tissues were either frozen crushed in the rapid controlled rate freezer (NSF grant support to MM .or native disintegrated with ultrasonicator (Branson Ultrasonic, Danbury, CT, USA).
For selection and in vitro evolution, the HER-2 receptors were extracted from the human HER-2+ ovarian cancer cells by immunoprecipitation of rapidly frozen, crushed, thawed, and lyophilized.
The cells and tissues were either frozen crushed in the rapid controlled rate freezer (the NSF grant support to MM .or native disintegrated with ultrasonicator (Branson Ultrasonic, Danbury, CT, USA).
For selection and in vitro evolution, the HER-2 receptors were extracted from the human HER-2+ breast cancer cells by immunoprecipitation of rapidly frozen, crushed, thawed, and lyophilized.
Temperature was maintained by addition of frozen, crushed DMEM to the cell chambers.
Pericarp tissues from the five sampling points were immediately frozen, crushed in liquid nitrogen, and stored at −80 °C for RNA extraction, small RNA and degradome sequencing.
Tissue samples were cut into 30-40 μg pieces for RNA extraction, snap frozen, crushed between cryogenically cooled steel blocks, and homogenised in 2 ml TRIreagent (Molecular Research Center, Cincinnati, OH, USA) using a Mini-Bead-Beater-8 (BioSpec Products, Bartlesville, OK, USA).
