Sentence examples for crude virus from inspiring English sources
Crude virus suspensions were obtained by freeze-thawing the plates thrice and removal of cell debris by centrifugation for 15 min at 2800 rpm.
Crude virus suspensions for titer determination were obtained by freeze-thawing the samples thrice and removing cell debris by centrifugation at 2800 rpm for 15 min.
The total volume of crude virus stock was quantified and inactivated by mixing with 37% formalin at a 1/4000 ratio, as described previously [ 21, 22], except that inactivation was maintained at 4°C for 1 week with stirring.
In order to confirm the impaired growth of the oversized virus carrying the BAC and stuffer sequences, we performed single-step growth assay using the crude virus preparations harvested in Fig 5b.
Crude virus was filtered through 0.45 μm PVDF filters (Millipore, Billerica, MA, USA), concentrated by ultracentrifugation at 100 000 × g for 60 min at 4°C and stored at −80°C until further use.
The up-stream process was investigated and optimized, and the crude virus bulk was 20-fold concentrated using either 100- or 300-kDa cut-off dia-filtration membranes in tangential flow filter (TFF) cassettes.,, The purpose of the TFF process was to concentrate viral particles and remove cellular proteins.
Crude, virus-containing extracts (sap) from these infected plants as well as virus-free extracts from non-infected plants were used for mechanical inoculation of young Arabidopsis plants (5 leaves stage).
The crude virus-containing medium was centrifuged and filtrated with a sterile 0.45 µm pore size filter (Millipore, Billerica, MA).
Supermarketer plants were inoculated with purified virions and crude virus-containing extracts from infected plants respectively, 9 days after germination.
(B) Detection by Northern-blot analysis of PNRSV RNAs in cucumber-plant leaf, cotyledon, root and phloem inoculated with crude virus-containing extracts from infected plants (lanes 4, 5, 6 and 8 respectively).
The response may represent specific IgG reactivity to measles antigens or unspecific auto-antibodies targeting cellular components in the crude whole virus antigens commonly used in detection assays.
