Sentence examples for crude template from inspiring English sources

In fact, studies indicate that a relatively crude template is innately determined in most species.

Nevertheless, species interactions (e.g. predation, competition and parasitism) modify this crude template profoundly [ 47, 59, 60] and behaviour in turn can affect both stress tolerance and species interactions [ 16, 17, 61].

Amplification reactions contained 10 μl of SYBR Green PCR Master Mix Applied Biosystemss, Warrington, UK), 300 nM each primer, and 300 ng of the respective crude template DNA or 1 μL of water (negative control) in a final volume of 20 μL.

Crude DNA template was prepared as follows: A pure culture of S. sonnei was plated on Luria-Bertani agar and incubated overnight at 37°C.

Colony forming units (cfu) per dilution were established by triplicate plate counts and real-time PCR reactions included GBS crude lysate template containing between 105 and 10-1 cequivalentsequivalents

To achieve highly reproducible PCR amplification using a single blastocyst as the source of the crude DNA template, we first checked several carriers, including yeast tRNA (Ambion), yeast total RNA (Ambion), herring sperm DNA (Promega), and glycogen (Life Technologies), all of which have been used to precipitate very low amounts of DNA.

The second adaptation of our workflow was the optimization of a protocol to prepare crude gDNA templates from small pools of larvae.

The copy number of rDNA operons of targeted bacteria in crude DNA templates was determined by comparison with serially-diluted plasmid DNA standards run on the same plate.

Two rounds of PCR were performed to amplify a 580-bp region of the murine Ramp2 gene using crude DNA as template.

The presence or absence of HR in the target locus was assessed by PCR amplification of the junctional region between the 5′ arm (or 3′ arm) and the host genome using crude DNA as template.

Finally, PCR was performed using each crude DNA sample as template to specifically amplify a 580-bp region corresponding to exon 1 of murine Ramp2.