Sentence examples for crude specimen from inspiring English sources

As shown in Figure 1, the amplification curves for 5 μl of extracted specimens were nearly identical to the curves generated when either 1 μl or 2.5 μl of crude specimen was analyzed.

However, in each case where 5 μl of crude clinical sample was analyzed, the internal controls either failed to amplify efficiently, or did not amplify at all indicating that the fundamental chemistry of PCR was negatively affected by something in the crude specimen, but that a significant amount of the crude specimen was required to be added to the reaction for such a negative impact to occur.

Although both 1 μl and 2.5 μl of crude specimen had performed adequately in PCR in our initial feasibility study, as shown in Figures 1A and 1B, we chose to use 1 μl for the remainder of the study for the reason that such a volume would carry over a smaller amount of potentially inhibiting factors, if any, in the clinical specimen.

Performing PCR on crude specimens does not require any sacrifice of specificity and requires only a minor sacrifice of assay sensitivity.

Crude specimens of approximately 50 g of environmental dirt, sewage disposal, or cattle waste were each collected in a sterile sample collection tube (100 ml).

The enhancer can be used for amplification of various DNA templates, including those which are GC-rich and present in crude specimens, where other enhancers such as DMSO or betaine fail.

Simultaneous to this, samples of those same three clinical specimens were also analyzed by real-time PCR, using 5, 2.5 and 1 μl of crude, unextracted specimen combined with water (if necessary) to achieve a final volume of 5 μl.

This optimized assay can process an entire crude BM specimen in less than 1 hour and results are analyzed on just one slide.

But, with something of vigorous over-emphasis, it has yet remarkable freshness and vivacity, and the "Wolf" himself is a strong conception, a cruder and harder specimen of a range of characters of which Turgenieff's Bazaroff is the greatest.

We investigated whether it would be feasible to detect HSV DNA in crude (unextracted) clinical specimens using the same Real-Time PCR reagents and methods currently utilized in our laboratory.

With preliminary evidence that crude, unextracted clinical swab specimens, when used in the proper amounts, are adequate for direct PCR analysis, we sought to determine the repeatability of this finding.