Sentence examples for crude serum from inspiring English sources

The immunoglobulin fraction of the antibody specific to LND was isolated from the crude serum sample of rabbit No. 2 by precipitation with saturated ammonium sulphate solution followed by purification on protein A column using affinity chromatography.

Western blot analysis of acid-extracted wild-type histones using crude serum compared to pre-immune serum demonstrated that this mark exists in vivo (data not shown).

To further corroborate this finding and characterize this novel mark, α-H2BK37me2 antibody was affinity purified from crude serum and peptide competition analysis was completed using acid-extracted wild-type H2B, H2B K37andand H2B K123R mutant histone samples.

To remove non-specific antibodies that might react with the BSA domain of the conjugate, the crude serum was first absorbed with CNBr-activated sepharose 4B resin (Amersham) to which BSA had covalently attached.

Approximately 50 µg of crude serum was dissolved in SDS-PAGE loading buffer, heated to 99°C in a thermal cycler (Eppendorf, Westbury, NY), and resolved on a 4 20% polyacrylamide-SDS gradient gel (Pierce, Rockford, IL).

After clearance by centrifugation, the crude serum obtained from the rabbits was used for immunological studies.

Interestingly the three samples, b, h, m, also gave lower results by band-shift when crude serums were assayed without PCA treatment (Fig. 4A).

The following primary antibodies were used: mouse 20H5 anti-centrin (1∶100, gift of Dr. Salisbury, Mayo Clinic, Rochester, MN), mouse 12G10 anti α-tubulin (1∶10, from University of Iowa, Developmental Studies Hybridoma Bank), rat purified (by affinity column) anti-CCTα (1∶10) (this work), crude rat serum anti-CCTα (1∶50) [29] and crude rat serum anti-CCTδ (1∶30) [1∶30

This study indicates that the high-capacity prototype matrix is a feasible and potentially cost-effective alternative for the direct capture of antibodies from crude ovine serum and may therefore also be applicable to the purification of other complex industrial feedstocks such as transgenic milk or monoclonal antibodies expressed using recombinant technologies.

Testosterone was measured in crude rat serum, which is reliable for comparison among groups, but matrix effects cause uncertainties with respect to absolute values.

This shows that although it is a crude tool, serum virus level may be used as an indicator of PMWS status on herd level, provided that the pigs are sampled at an appropriate time, i.e. during the first weeks in the finishing herd.