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From the report of Ng et al. [23] showed that, use only 20% (w/w) of crude load provided the highest recovery (70.53%) and PF (13.1) in partitioning cyclodextrin glycosyltransferase derived from Bacillus cereus from rotten potato by ATPS.
The previous study of Amid et al. [22] on the partitioning of serine protease from mango peel using an alcohol/salt ATPS found that 20% (w/w) crude load indicated maximum capacity on the basis of 10 g of ATPS with the yield of 95.8% and 11-fold of purity.
Lipase (Tan et al. 2015) was successfully recovered from the polymer-rich top phase in the system of EOPO 3900 with 48.5 % (w/w) tie-line length, VR of 2.3 and 20%% (w/w) of crude load at pH 7 in primary ATPS (Show et al. 2012).
The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel.
The increase in crude load is an advantage in the recovery process of this ATPS technique.
Moreover, the selectivity and the yield of 20% (w/v) crude load ATPS were 122.3 and 94.3%, respectively.
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"The Libyan National Oil Corporation wishes to make it clear that the crude oil loaded upon the vessel 'Morning Glory' is the property of NOC and its partners," the company said in a statement before the vessel's takeover by the rebels.
ATPS studies are performed by varying the crude loads, which can reach proportions of up to 50% (w/v).
Figure 1 represents the elution profile of the crude enzyme loaded on DEAE-cellulose anion exchanger column where linear gradient of 0.2 1.0 M NaCl was used as eluent.
Two optimal point with high enzyme partitioning factor 10.97 and yield 79.95 (including 10% PEG, 12% Na2SO4, 50% ligand, pH 8 and 6% crude enzyme loading) and high specific activity in top phase 42.21 (including 14.73% PEG, 8.02% Na2SO4, 28.43% ligand, pH 7.7 and 6.08% crude enzyme loading) were attained.
The influence of some experimental variables such as PEG (10 18%w/w), sodium sulfate (8 12%), PEG-IDA-Cu2+ concentration (0 50% w/w of total PEG), pH of system (4 8) and crude enzyme loading (6 18%w/w) on xylanase and total protein partitioning coefficient, enzyme yield and enzyme specific activity were systematically evaluated.
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Justyna Jupowicz-Kozak
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