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Lipids were removed from the crude egg extract using the modified Bligh and Dyer method [18], and then 30 mg of crude egg extract were dissolved in 3 ml of 1∶2 chloroform/methanol and vortexed, and 1 ml of chloroform was added and vortexed.
In our research, the termite egg extracts became easier to precipitate and lost their activity during storage as purification proceeded (Fig. S1), while crude egg extracts maintained their activity for a long time.
Most techniques detect IgG, IgM or IgE against soluble worm antigen or crude egg antigen by ELISA, indirect hemagglutination or immunofluorescence [ 1, 2].
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Crude shrimp egg 80S ribosomes were prepared from dried, frozen cysts as previously described (Iwasaki and Kaziro, 1979; Thiele et al., 1985) with some modifications.
Open image in new window Fig. 5 Bioassay of crude antibiotic on egg hatching and juvenile mortality of M. incognita.
Crude exact of egg white was dissolved in PBS and 20 μL was added to the wells of agar plate containing Micrococcus lysodeikticus.
In addition, in order to ascertain the suitability of infected urine samples and parasite eggs for immunolocalization studies on ShSSA, this study was conducted with a view to determine the reactivity of MAb to crude antigens from eggs and urine of study subjects.
Eggs treated with B. subtilis strain Bbv 57 crude antibiotic showed poor egg hatching.
From the lowest to the highest category of egg consumption, crude incidence rates of diabetes were 35.8, 41.3, 42.7, 46.8, 62.4, and 67.0 cases per 10,000 person-years in the PHS I.
Crude, off-colour objectification or egg on toast and a nice bunch of daffodils?
Crude lipid contents in sturgeon eggs vary from 12.5 to 27%% wet weight and are high in the n-3 fatty acids, such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids (Bledsoe et al. 2003; Gessner et al. 2002).
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