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The Mangala crude 1 (entry 33, Table 3) and Mangala crude 2 (entry 27, Table 4) have been subjected to NMR analysis to estimate the hydrogen content in them.
The diluted cell lysate (30 ml, 1 volume) was then applied onto a HisTrap™FF crude (1 ml, GE Healthcare, Sweden).
After removal of the solvent, the residue was extracted with pentane yielding crude 1. Distillation under reduced pressure (89 °C, 0.026 mbar) yielded 80%% pure product (12.30 g, 35.9 mmol).
His6-tagged rBipA was purified on a AKTA FPLC system using affinity chromatography on a HisTrap FF crude 1 mL column prepacked with Ni Sepharose 6 Fast Flow GE Healthcaree, Uppsala, Sweden).
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The pellet diet consisted of 56.17% carbohydrate, 22.02% crude protein, 4.25% crude oil, 3.25% crude fibre, 2.46% glucose, 0.8% calcium, 0.6% phosphorus, and 1.8% vitamins.
The diets contained approximately 49, 54, 58 or 63% crude protein and 11, 16, 20, 23 or 28% crude lipid (6 23% starch).
crude oil 5.1 2.4 Conv.
o The mixed company expects to produce approximately 240,000 b/d of extra heavy crude (8.5B0 API).
The 1917 V-8, a crude 288-cubic-inch, 36-horsepower engine, was discontinued the next year.
In one key change Labour is to pledge an end to the "crude 15-minute slots" of care visits.
The crude 1-year prevalence of migraine was 34.7 % (17.5 % definite, 17.2 % probable).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com